In WB, the pore size of the membrane has a profound effect on the efficiency and precision of protein transfer and detection. With its advantages and disadvantages, a careful evaluation of experimental needs and priorities, balancing factors such as protein size, detection sensitivity, throughput, and workflow efficiency, is crucial when selecting the appropriate pore-size membrane for WB experiments.
We're all about setting you up for success in the lab, so we've put together a detailed explanation of the difference between small and large pore-size WB membranes and how they affect your results.
Grab the explanation and let's ace those experiments together!