Blue/Green LED Technology
The revolution for safe
nucleic acid gel documentation
The Danger of UV-Light
UV-light use just a single wavelength for the visualization of DNA. Red and green DNA dyes, like ethidium bromide or the MIDORIGreen dyes have usually a good emission in the UV-light spectra. The results are DNA bands with a sufficient intensity. However, UV-light is dangerous for the user and for the DNA. Just 30 sec of UV-light exposure significantly reduces the cloning efficiency and has consequences for other downstream applications. For this reason, the visualization of DNA using UV-light is not the state-of-the-art-method anymore.
Blue/Green LED - The Revolution
Instead of using a single wavelength, the Blue/Green LED technology use a combination of wavelengths in a spectrum of light of 470 nm to 520 nm. This Blue/Green light is able to excite all common red and green DNA dyes with a very high intensity. The very high intensity can be achieved by the accumulated energy absorption of the dyes.